Biolistic DNA Delivery in Turfgrass Embryonic Callus Initiated from Mature Seeds
We describe a protocol for the establishment and preparation of creeping bentgrass (Agrostis stolonifera L.) cultivar “Penn A-4” embryonic calli, biolistic transformation, selection, and regeneration of transgenic plants. The embryonic callus is initiated from mature seeds, maintained by visual selection under the dissecting microscope and subjected to bombardment with plasmid DNA containing a bialaphos-resistance (bar) gene. PCR, Southern, and Northern blot analyses are used to confirm the transgene integration and expression.
Methods in Molecular Biology
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Zhou, Man; Zhao, Junming; Li, Dayong; Yuan, Shuangrong; Yuan, Ning; Li, Zhigang; Jia, Haiyan; Gao, Fangyuan; San, Bekir; Hu, Qian; and Luo, Hong, "Biolistic DNA Delivery in Turfgrass Embryonic Callus Initiated from Mature Seeds" (2020). Kean Publications. 1286.