Mutational analysis of the functional motifs of the DEAD-box RNA helicase Me31B/DDX6 in Drosophila germline development
Document Type
Article
Publication Date
7-1-2023
Abstract
Me31B/DDX6 is a DEAD-box family RNA helicase playing roles in post-transcriptional RNA regulation in different cell types and species. Despite the known motifs/domains of Me31B, the in vivo functions of the motifs remain unclear. Here, we used the Drosophila germline as a model and used CRISPR to mutate the key Me31B motifs/domains: helicase domain, N-terminal domain, C-terminal domain and FDF-binding motif. Then, we performed screening characterization on the mutants and report the effects of the mutations on the Drosophila germline, on processes such as fertility, oogenesis, embryo patterning, germline mRNA regulation and Me31B protein expression. The study indicates that the Me31B motifs contribute different functions to the protein and are needed for proper germline development, providing insights into the in vivo working mechanism of the helicase.
Publication Title
FEBS Letters
First Page Number
1848
Last Page Number
1867
DOI
10.1002/1873-3468.14668
Recommended Citation
Kara, Evan; McCambridge, Aidan; Proffer, Megan; Dilts, Carol; Pumnea, Brooke; Eshak, John; Smith, Korey A.; Fielder, Isaac; Doyle, Dominique A.; Ortega, Bianca M.; Mukatash, Yousif; Malik, Noor; Mohammed, Ammaar R.; Govani, Deep; Niepielko, Matthew G.; and Gao, Ming, "Mutational analysis of the functional motifs of the DEAD-box RNA helicase Me31B/DDX6 in Drosophila germline development" (2023). Kean Publications. 113.
https://digitalcommons.kean.edu/keanpublications/113