Title

Biolistic DNA Delivery in Turfgrass Embryonic Callus Initiated from Mature Seeds

Authors

Man Zhou, Wenzhou-Kean University
Junming Zhao, Clemson University
Dayong Li, Clemson University
Shuangrong Yuan, Clemson University
Ning Yuan, Clemson University
Zhigang Li, Clemson University
Haiyan Jia, Clemson University
Fangyuan Gao, Clemson University
Bekir San, Clemson University
Qian Hu, Clemson University
Hong Luo, Clemson University

Document Type

Article

Publication Date

1-1-2020

Abstract

We describe a protocol for the establishment and preparation of creeping bentgrass (Agrostis stolonifera L.) cultivar “Penn A-4” embryonic calli, biolistic transformation, selection, and regeneration of transgenic plants. The embryonic callus is initiated from mature seeds, maintained by visual selection under the dissecting microscope and subjected to bombardment with plasmid DNA containing a bialaphos-resistance (bar) gene. PCR, Southern, and Northern blot analyses are used to confirm the transgene integration and expression.

Publication Title

Methods in Molecular Biology

First Page Number

251

Last Page Number

261

DOI

10.1007/978-1-0716-0356-7_13

Recommended Citation

Zhou, Man; Zhao, Junming; Li, Dayong; Yuan, Shuangrong; Yuan, Ning; Li, Zhigang; Jia, Haiyan; Gao, Fangyuan; San, Bekir; Hu, Qian; and Luo, Hong, "Biolistic DNA Delivery in Turfgrass Embryonic Callus Initiated from Mature Seeds" (2020). Kean Publications. 1286.
https://digitalcommons.kean.edu/keanpublications/1286