Biolistic DNA Delivery in Turfgrass Embryonic Callus Initiated from Mature Seeds
Document Type
Article
Publication Date
1-1-2020
Abstract
We describe a protocol for the establishment and preparation of creeping bentgrass (Agrostis stolonifera L.) cultivar “Penn A-4” embryonic calli, biolistic transformation, selection, and regeneration of transgenic plants. The embryonic callus is initiated from mature seeds, maintained by visual selection under the dissecting microscope and subjected to bombardment with plasmid DNA containing a bialaphos-resistance (bar) gene. PCR, Southern, and Northern blot analyses are used to confirm the transgene integration and expression.
Publication Title
Methods in Molecular Biology
First Page Number
251
Last Page Number
261
DOI
10.1007/978-1-0716-0356-7_13
Recommended Citation
Zhou, Man; Zhao, Junming; Li, Dayong; Yuan, Shuangrong; Yuan, Ning; Li, Zhigang; Jia, Haiyan; Gao, Fangyuan; San, Bekir; Hu, Qian; and Luo, Hong, "Biolistic DNA Delivery in Turfgrass Embryonic Callus Initiated from Mature Seeds" (2020). Kean Publications. 1286.
https://digitalcommons.kean.edu/keanpublications/1286