An Arabidopsis thaliana leucine-rich repeat protein harbors an adenylyl cyclase catalytic center and affects responses to pathogens

Authors

Chantal Bianchet, Università degli Studi di Perugia
Aloysius Wong, Wenzhou-Kean University
Mara Quaglia, Università degli Studi di Perugia
May Alqurashi, King Abdullah University of Science and Technology
Chris Gehring, Università degli Studi di Perugia
Vardis Ntoukakis, Faculty of Science, Engineering and Medicine
Stefania Pasqualini, Università degli Studi di Perugia

Document Type

Article

Publication Date

1-1-2019

Abstract

Adenylyl cyclases (ACs) catalyze the formation of the second messenger cAMP from ATP. Here we report the characterization of an Arabidopsis thaliana leucine-rich repeat (LRR) protein (At3g14460; AtLRRAC1) as an adenylyl cyclase. Using an AC-specific search motif supported by computational assessments of protein models we identify an AC catalytic center within the N-terminus and demonstrate that AtLRRAC1 can generate cAMP in vitro. Knock-out mutants of AtLRRAC1 have compromised immune responses to the biotrophic fungus Golovinomyces orontii and the hemibiotrophic bacteria Pseudomonas syringae, but not against the necrotrophic fungus Botrytis cinerea. These findings are consistent with a role of cAMP-dependent pathways in the defense against biotrophic and hemibiotrophic plant pathogens.

Publication Title

Journal of Plant Physiology

First Page Number

12

Last Page Number

22

DOI

10.1016/j.jplph.2018.10.025

Recommended Citation

Bianchet, Chantal; Wong, Aloysius; Quaglia, Mara; Alqurashi, May; Gehring, Chris; Ntoukakis, Vardis; and Pasqualini, Stefania, "An Arabidopsis thaliana leucine-rich repeat protein harbors an adenylyl cyclase catalytic center and affects responses to pathogens" (2019). Kean Publications. 1431.
https://digitalcommons.kean.edu/keanpublications/1431