Determining annealing temperatures for polymerase chain reaction
Document Type
Article
Publication Date
4-1-2012
Abstract
The polymerase chain reaction (PCR) is a common technique used in high school and undergraduate science teaching. Students often do not fully comprehend the underlying principles of the technique and how optimization of the protocol affects the outcome and analysis. In this molecular biology laboratory, students learn the steps of PCR with an emphasis on primer composition and annealing temperature, which they manipulate to test the effect on successful DNA amplification. Students design experiments to test their hypotheses promoting a discovery-based approach to laboratory teaching and development of criticalthinking and reasoning skills. © 2012 by National Association of Biology Teachers. All rights reserved.
Publication Title
American Biology Teacher
First Page Number
256
Last Page Number
260
DOI
10.1525/abt.2012.74.4.9
Recommended Citation
Porta, Angela R. and Enners, Edward, "Determining annealing temperatures for polymerase chain reaction" (2012). Kean Publications. 2177.
https://digitalcommons.kean.edu/keanpublications/2177