Using a novel approach - recombineering - to generate odf2 null alleles

Document Type

Article

Publication Date

2-1-2012

Abstract

This article uses a real-life example to illustrate the concept and methodology of recombineering, a revolutionary genetic engineering technique based on phage-mediated homologous recombination. A step-by-step approach is presented along with a flow diagram, from obtaining gene-harboring BACs to the in vitro generation of a conditional null allele. This method can be used to target any gene at any position, without the knowledge or use of any restriction site. The extensive applicability of recombineering to gene manipulation is discussed. © 2012 Higher Education Press and Springer-Verlag Berlin Heidelberg.

Publication Title

Frontiers in Biology

First Page Number

83

Last Page Number

91

DOI

10.1007/s11515-011-1176-9

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